RNA
E1A2

Part:BBa_K273021

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-17)

E1A2

Antisense RNA against the formation of pyruvate dehydrogenase complex (PDC). Target is pyruvate dehydrogenase E1 (EC 1.2.4.1), in partuclar the alfa subunit (A). Antisense RNA approach #2.

Will be transcribed together with the BioBrick interface and may affect the performance of the antisense RNA molecule.

EXYZ
EX: E1 enzyme subunit of the PDC complex
Y: a=Alpha subunit of the particular enzyme subunit
b=beta subunit of the particular enzyme subunit
Z: # of antisense RNA approach

Usage and Biology

Antisense RNA function supposed to fuction through either Shine Dalgarno blocking and/or RNA elongation blocking.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None